Research Article
CCR7 Receptor Expression in Mono-MAC-1 Cells: Modulation by Liver X Receptor α Activation and Prostaglandin E2
Figure 6
The role of cAMP and alternative E/E signaling pathways in PG- and T0901317-induced CCR7 mRNA expression and function. (a) MM-1 cells were treated for 8 h (filled black) and 24 h (filled gray) with 100 μM forskolin alone or in combination with 1 μM T0901317. Total RNA was extracted and CCR7 transcripts were detected using real-time qPCR. (b) Chemotaxis assays in response to 300 ng/mL CCL19 (filled black) or CCL21 (filled gray) were performed in MM-1 cells treated with the indicated concentrations of H-89, Ly294002, and PD98059 before the addition of 1 μM PG and T0901317 for 48 h. The mean number of spontaneously migrating cells (that migrated to media alone) was subtracted from the number of cells that migrated in response to CCL19 or CCL21. Data represent mean ± SD of three independent experiments. , .
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