EOLA1 Inhibits Lipopolysaccharide-Induced Vascular Cell Adhesion Molecule-1 Expression by Association with MT2A in ECV304 Cells

Figure 3

Overexpression of EOLA1 prevents LPS-induced VCAM-1 production in ECV304 cells. ECV304 cells were transfected with pOPRSVI-EOLA1-EGFP and pCMV-LacI plasmids and induced by IPTG. (a) Overexpression of EOLA1 was demonstrated by Western blotting analysis with anti-EOLA1 antibody after adding IPTG for 48 h. (b) The induced cells with IPTG were tested for their ability to produce VCAM-1 upon LPS stimulation (100 ng/mL) for 6 h using a commercial ELISA kit. Data represent the mean ± SD of three independent experiments ().

(a)

(b)