Lipopolysaccharide from Rhodobacter sphaeroides Attenuates Microglia-Mediated Inflammation and Phagocytosis and Directs Regulatory T Cell Response

Figure 3

LPS-Rs ameliorates microglia-mediated neuronal insults. BV2 cells were differentially treated in absence and presence of LPS-Rs followed by LPS treatment as indicated. Later the differentiated neuro2a cells were cocultured with BV2 cells and neuronal viability was measured using MTT and morphological observation (b, c, d). Supernatants from conditioned microglial cells were used to treat differentiated neuro2a cells and the Bax : Bcl2 ratio was measured following Western blot (e). BV2 cells were transfected with control or TLR4 siRNA and TLR4 silencing was assayed by Western blot (f). TLR4 silencing suppressed LPS induced neuronal cell death (g). The error bars represent the mean ± SEM from three independent experiments ( versus control and versus LPS). UN: untreated; LPS: lipopolysaccharide from Escherichia coli 055 : B5 (1 µg/mL); RS: lipopolysaccharide from Rhodobacter sphaeroides (0.5–5 µg/mL).