Lipopolysaccharide from Rhodobacter sphaeroides Attenuates Microglia-Mediated Inflammation and Phagocytosis and Directs Regulatory T Cell Response

Figure 5

LPS-Rs inhibit costimulatory molecules and leukocyte trafficking factors required for T cell interaction and infiltration. BV2 microglia were pretreated with LPS-Rs for 2 hrs followed by LPS treatment for 6 hrs and the mRNA expression of macrophage activation markers: MHC-II, CD40, CD80, and CD86 (a) and chemokines and chemokine receptors (MIP-1α, CCL5, and CCR5) (b) were detected by RT-PCR. The error bars represent the mean ± SEM from three independent experiments ( versus control and versus LPS). UN: untreated; LPS: lipopolysaccharide from Escherichia coli 055 : B5 (1 µg/mL); RS: lipopolysaccharide from Rhodobacter sphaeroides (0.5–5 µg/mL).

(a)

(b)