Table 2: Relative estrogenicity of androstene hormones.

HormoneEC50 (nM)EstrogenicityP value

17β-Estradiol0.04611
Δ5-Androstene-3β, 17β-diol131/282<0.001
Δ5-Androstene-3β, 7β, 17β-triol271/587<0.001
DHEA1631/3543<0.001
Δ5-Androstene-3β, 17α-diol3501/7609<0.001

The EC50 was calculated as the 50% activation point. The estrogenicity is the 17β-estradiol EC50 divided by the androstene hormone EC50. This data does not normalize the cellular uptake of androstene hormones.