Structural Stereochemistry of Androstene Hormones Determines Interactions with Human Androgen, Estrogen, and Glucocorticoid Receptors

Table 2

Relative estrogenicity of androstene hormones.

Hormone	EC50 (nM)	Estrogenicity	P value
17β-Estradiol	0.046	1	1
Δ^5-Androstene-3β, 17β-diol	13	1/282	<0.001
Δ^5-Androstene-3β, 7β, 17β-triol	27	1/587	<0.001
DHEA	163	1/3543	<0.001
Δ^5-Androstene-3β, 17α-diol	350	1/7609	<0.001

The EC₅₀ was calculated as the 50% activation point. The estrogenicity is the 17β-estradiol EC₅₀ divided by the androstene hormone EC₅₀. This data does not normalize the cellular uptake of androstene hormones.