Synthesis, Characterization, and Antimicrobial Activity of a Novel Trisazo Dye from 3-Amino-4H-thieno[3,4-c][1]benzopyran-4-one

Table 1

Important HMBC interactions in compound 7; ¹H and ¹³C chemical shifts (δ/ppm) in DMSO- as the solvent (25°C).

Compound 7			Compound 7
C atom	δ^13C	HMBC (H → C)	C atom	δ^13C	HMBC (H → C)
7	129.6	H-8 (8.32)	9a′′	130.5	H-8′′ (8.59), H-7′′ (8.27), H-6′′ (7.97)
9	126.8	H-1 (7.67)	1′′′	115.4	H-9′′′ (8.83), H-1′′′ (7.58)
9a	131.6	H-1 (7.67)	3′′′	135.8	H-9′′′ (8.83), H-7′′′ (8.15), H-1′′′ (7.58)
9b	157.2	H-8 (8.32), H-6 (7.99)	4′′′	164.2	H-7′′′ (8.15)
1′′	115.3	H-1′′ (7.50)	5a′′′	155.3	H-9′′′ (8.83), H-7′′′ (8.15)
5a′′	155.4	H-6′′ (7.97)	6′′′	117.6	H-7′′′ (8.15)
7′′	129.4	H-9′′ (9.01), H-6′′ (7.97)	9′′′	127.6	H-8′′′ (8.46), H-1′′′ (7.58)
8′′	125.8	H-8′′ (8.59)	9a′′′	129.7	H-1′′′ (7.58)
9′′	127.6	H-1′′ (7.50)