A Comparison of Imaging Techniques to Monitor Tumor Growth and Cancer Progression in Living Animals
Figure 2
Tumor growth monitored in vivo by optical imaging. (a) Signal to background ratio is similar for BLI (B16-luc, left) and FLI (B16-RFP, right) in vitro. Results are shown as the mean and SD of 4 replicate wells from 2 independent experiments. (b) B16-luc and B16-RFP tumors grow equally in vivo. Following subcutaneous injection of cell lines into mice ( mice for each cell line), tumor volume was calculated from caliper measurements. Four tumors were measured for each cell line. NS, no significant difference between B16-luc and B16-RFP (, test according to [13]). (c) BLI is more sensitive for tumor detection in vivo. B16-luc or B16-RFP cells were injected subcutaneously. Mice ( mice each for BLI and FLI) were shaved and imaged. Four tumors were measured for each cell line. The dotted line represents the detection threshold calculated based on control tumors not expressing the relevant reporter gene. *, significant difference between B16-luc and B16-RFP (, test according to [13]).
