Research Article

Evaluation of Nonradiative Clinical Imaging Techniques for the Longitudinal Assessment of Tumour Growth in Murine CT26 Colon Carcinoma

Figure 5

Assessment of perfusion and permeability data in vivo by MRI and ex vivo by immunofluorescence. (a) MRI image of CT26 tumour (white arrow) before Gd contrast agent injection and (b) MRI image of CT26 tumour (white arrow) after Gd contrast agent injection. (c) Representation of / as a function of time after injection of Gd contrast agent in the edge and the centre of ectopic CT26 tumours. Ex vivo immunofluorescence in the edge (d) and in the centre (e) of ectopic CT26 tumour showing endothelial cells (CD31-FITC, green labeling) and perfused tumour cells (Hoechst 33342, blue labeling). (f) Intensity of perfused tumour cells in the edge and in the centre of ectopic tumour cells quantified by ImageJ.
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