Comparison of Folate Receptor Targeted Optical Contrast Agents for Intraoperative Molecular Imaging

Figure 2

KB, HeLa, and TC1 cells folate receptor α staining (a) followed by KB, HeLa, and TC1 cells cocultured with fluorescent probes and imaged at 200x using a confocal microscope (b) and (c). Fluorescence was demonstrated in the viable cancer cells. (a) FRα staining (from left to right) of KB cells positive for folate receptor α. HeLa FRα staining also positive for folate receptors. TC1 cells staining negative for FRα. (b) Fluorescent imaging (bottom left) highlights KB cells fluorescing with EC17 fluorescence dye at 2.50 μM, followed by fluorescent imaging of HeLa cells with EC17. Imaging (top right) exhibits negative coculture for TC1. (c) Near-infrared fluorescent imaging (bottom left) displays KB cells fluorescing with OTL38 fluorescence dye at 2.50 μM. The following image demonstrates HeLa cells fluorescing with EC17. TC1 negative for OTL38 fluorescence.

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