Research Article
Phenotypic and Genotypic Antimicrobial Resistance Profiles of Campylobacter jejuni Isolated from Cattle, Sheep, and Free-Range Poultry Faeces
Table 2
Primers and probes sequences.
| | Target | Name | Sequences () | C (nM) |
| | gyrA | gyr-Fw | GGGTGCTGTTATAGGTCGTTATCA | 900 | | gyr-Rv | TTGAGCCATTCTAACCAAAGCAT | 900 | | Probe-gyr-S | HEX – CAT[+G]GAGAT[+A][+C][+A]GC[+A]GTTT – BHQ1 | 150 | | Probe-gyr-R | FAM – CATGGAGATATAGCAGTTT – MGB | 150 |
| | 23S | 23S-Fw | CAGTGAAATTGTAGTGGAGGTGAAA | 900 | | 23S-Rv | TTCTTATCCAAATAGCAGTGTCAAGCT | 900 | | Probe-23S-S | HEX – CGGGGTC[+T][+T][+T]CCGTCTTG – BHQ1 | 100 | | Probe-23S-R | FAM – CGGGGTC[+T][+C][+T]CCGTCTTG – BHQ1 | 200 |
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LNA nucleotides are indicated by a + symbol and in brackets; SNPs are underlined
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