Antifungal Activity of Selected Indigenous Pseudomonas and Bacillus from the Soybean Rhizosphere

Table 5

Inoculation of soybean seeds with the potential biocontrol agents, response of plant growth in soil infested with Pythium ultimum.

Treatment	% Disease suppression*	Emergence rate (%)^†	Shoot dry wt. (g/plant)^‡	% Nitrogen/plant ^§
Control	85.6 ± 19.4^cd	93 ± 01^b	2.31 ± 0.30^a	1.72 ± 0.17^a
BNM296	95.88 ± 10.1^d	97 ± 03^b	2.72 ± 0.43^b	2.62 ± 0.19^b
BNM297	86.6 ± 12.6^cd	90 ± 01^b	2.39 ± 0.28^ab	1.70 ± 0.25^a
BNM340	86.6 ± 12.6^cd	93 ± 01^b	2.38 ± 0.32^ab	1.74 ± 0.16^a
P	0.20 ± 16.7^a	31 ± 11^a	1.53 ± 0.19^c	1.66 ± 0.20^a
P+BNM296	53.7 ± 26.4^b	90 ± 04^b	2.28 ± 0.42^ab	2.49 ± 0.21^b
P+BNM297	1.20 ± 21.4^a	33 ± 16^a	0.60 ± 0.73^d	1.68 ± 0.13^a
P+BNM340	74.07 ± 22.0^c	89 ± 06^b	2.54 ± 0.22^ab	1.85 ± 0.13^a

^∗Percent disease suppression was calculated using [(𝐴−𝐵)/𝐴]× 100, where 𝐴 = root rot severity index exhibited by the control treatment inoculated with the pathogen alone and 𝐵 = root rot severity index exhibited by plants treated with both the pathogen and the bacterial antagonists. Control = non-inoculated seeds in non-infested substrate; P = substrate infested with P. ultimum; BNM296, BNM297, and BNM340; seeds inoculated with P. fluorescens BNM296, P. fluorescens BNM297 or B. amyloliquefaciens BNM340 in non-infested substrate; and P+BNM296, P+BNM297 and P+BNM340, seeds inoculated with BNM296, BNM297, or BNM340 in substrate infested with P. ultimum. Means within columns followed by the same letters do not differ significantly (P < 0.10, MSD = 17.9). ^†Means ± SE (P < 0 .05, MSD = 0.2). ^‡Means ± SE (P < 0 .05, MSD = 0.73). ^§Means ± SE (P < 0 .1, MSD = 0.44) all according to the LSD test using the Infostat software.