Research Article

Pectinolytic and Cellulolytic Enzymes Enhance Fusarium compactum Virulence on Tubercles Infection of Egyptian Broomrape

Figure 3

(a)–(e) Adding pectinase and cellulase to chopped F. compactum mycelia enhanced fungal virulence on broomrape. Roots of tomato plants infested with broomrape were sprayed to run-off with 10 ml of 106 propagules ml−1 of chopped mycelia in 0.01% Tween 20. (a) A total of 20 U solutions of pectinase and cellulase (1 : 1) were used as a control treatment. (b) 1.4 × 106 propagules ml−1 homogenized mycelia alone, (c) mycelia plus 20 U pectinase, (d) mycelia plus 20 U cellulase, and (e) 10 U pectinase and 10 U cellulase (20 U total enzyme)  ml−1 of homogenized mycelial suspension. Each plant bore an average of healthy tubercles at inoculation. (f) The graphical representation of (a) to (e). □ Pectinase (10 U ml−1) plus cellulase (10 U ml−1), ◊F. compactum (1.4 × 106 propagules ml−1), ∘ F. compactum plus cellulase (20 U ml−1), ∆ F. compactum plus pectinase (20 U ml−1), ⊞F. compactum plus cellulase (10 U ml−1) and pectinase (10 U ml−1).
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