Boric Acid Disturbs Cell Wall Synthesis in Saccharomyces cerevisiae
Figure 5
Electron micrographs of YPH499 cells grown in the presence of 0.3% and 0.4% BA. Cell wall chitin appears electron translucent. (a) No BA. Left: a mother-daughter pair; Middle: a close-up of the bud neck; Right: a completed trilaminar septum. Note the thin layer of chitin (primary septum; arrow) in the center of the structure. (b) Cells grown with 0.3% BA. Left: a mother-daughter pair; Middle: a close-up of the bud neck; Right: a completed septum. (c) Examples of other cell wall abnormalities in cells grown with 0.3% BA. Left: cell wall thickening and lacunae at the cell periphery; Middle: massive cell wall protuberances; Right: a septum forming at an ectopic location. (d) Cells grown with 0.4% BA. Left: a mother-daughter pair; Middle: a close-up of the bud neck; Right: a completed default septum. (e) Examples of other cell wall abnormalities in cells grown with 0.4% BA. Left: a chain of cells connected by default septa; Middle: a large cell wall structure spanning the entire length of the cell; Right: chitin-rich cell wall material deposited at the bud neck with two embedded aberrant septa (arrows).
