International Journal of Microbiology / 2011 / Article / Tab 1 / Research Article
Suitability of Commercial Transport Media for Biological Pathogens under Nonideal Conditions Table 1 Viability of B. anthracis , Y. pestis, and VEE. The numbers shown are percentages of total specimen loaded onto the rayon swab heads on day 0. In an effort to consolidate the large number of samples being extracted, VEE collection did not start until day 7 and persisted only until day 21. Boxes have been grayed out where there is discordance between bacterial and viral collection points. In addition, no data was taken for a sample after it displayed 0% viability for two successive time points, indicated by a (−).
Biothreat agent Storage temp. Storage Media Day 1 Day 3 Day 7 Day 14 Day 21 Day 28 Day 60 Bacillus anthracis −70°C PBST 3.8 ± 0.3 0.91± 0.13 5.0 ± 0.80 4.73 ± 0.91 1.76 ± 0.36 0.69 ± 0.1 3.48 ± 0.42 Amies 3.69 ± 0.4 2.55 ± 0.6 7.61 ± 1.6 13.5 ± 3.8 3.1 ± 0.7 4.26 ± 0.5 3.8 ± 0.6 Stuart 2.52 ± 0.5 2.92 ± 0.5 17.4 ± 3.6 9.83 ± 1.5 5.57 ± 0.7 7.97 ± 1.6 7.47 ± 1.1 Dry 0.9 ± 0.03 4.52 ± 0.6 2.61 ± 0.2 1.92 ± 0.3 5.72 ± 0.3 1.69 ± 0.1 6.46 ± 0.6 4°C PBST 4.7 ± 0.37 0.14 ± 0.03 0.08 ± 0.02 0.01 ± 0.001 0.87 ± 0.001 0.002 ± 0.001 0.005 ± 0.001 Amies 7.57 ± 0.4 0.85 ± 0.2 0.61 ± 0.1 0.05 ± 0.02 0.006 ± 0.001 0.004 ± 0.001 0.004 ± 0.001 Stuart 14.2 ± 3.57 2.25 ± 0.4 0.8 ± 0.07 0.3 ± 0.04 0.006 ± 0.001 0.002 ± 0.001 0.003 ± 0.001 Dry 1.25 ± 0.05 0.9 ± 0.06 0.006 ± 0.001 0.002 ± 0.001 0.002 ± 0.001 0.002 ± 0.001 0.003 ± 0.001 25°C PBST 73.1 ± 15.9 52.4 ± 5.94 114 ± 17 62.1 ± 6.32 79.6 ± 3.85 71.3 ± 4.37 56.7 ± 5.23 Amies 68.1 ± 14.8 102 ± 11.6 451 ± 22 372 ± 46 293 ± 39 298 ± 28 258 ± 11 Stuart 87.4 ± 2.61 122 ± 6 171 ± 23 162 ± 18 195 ± 28 215 ± 18 249 ± 22 Dry 7.93 ± 3.18 2.92 ± 0.7 0.8 ± 0.2 0.8 ± 0.1 2.81 ± 0.5 0.4 ± 0.04 0.6 ± 0.2 45°C PBST 0.01 ± 0.001 0.01 ± 0.001 0.01 ± 0.001 0.003 ± 0.001 0.004 ± 0.001 0.003 ± 0.001 >0.001 Amies 16.0 ± 2.0 2.47 ± 0.3 3.98 ± 0.7 1.98 ± 0.4 0.22 ± 0.06 0.22 ± 0.04 0.003 ± 0.001 Stuart 0.15 ± 0.02 0.01 ± 0.002 0.06 ± 0.01 >0.001 0.1 ± 0.01 0.005 ± 0.001 0.01 ± 0.002 Dry 0.06 ± 0.003 0.01 ± 0.003 0.002 ± 0.001 >0.001 >0.001 >0.001 >0.001 Yersinia pestis −70°C PBST 1.93 ± 0.8 0.1 ± 0.02 0.03 ± 0.004 0.06 ± 0.005 0.23 ± 0.03 0.07 ± 0.01 0.1 ± 0.01 Amies 2.13 ± 0.6 3.0 ± 0.2 1.4 ± 0.2 3.9 ± 0.3 2.8 ± 0.3 0.5 ± 0.04 1.7 ± 0.08 Stuart 2.1 ± 0.6 6.3 ± 1.1 3.1 ± 0.5 2.8 ± 0.6 4.7 ± 0.4 2.2 ± 0.8 2.4 ± 0.2 Dry 0.06 ± 0.01 0.4 ± 0.02 0.3 ± 0.05 0.6 ± 0.05 0.4 ± 0.03 0.3 ± 0.06 0.5 ± 0.01 4°C PBST 20.2 ± 2.2 7.8 ± 0.7 13.4 ± 1.8 4.8 ± 0.4 6.7 ± 0.5 3.8 ± 0.3 2.6 ± 0.2 Amies 49.5 ± 3.6 37.8 ± 2.5 54.2 ± 3.2 53.6 ± 4.6 50.8 ± 4.8 12.8 ± 1.2 10.0 ± 1.1 Stuart 41.3 ± 5.1 22.8 ± 1.2 32.8 ± 3.3 66.0 ± 6.2 51.8 ± 4.4 15.2 ± 1.3 32.0 ± 2.2 Dry 12.0 ± 1.1 4.1 ± 0.4 0.08 ± 0.02 0.02 ± 0.005 >0.001 >0.001 >0.001 25°C PBST 6.6 ± 0.8 4.7 ± 0.3 3.8 ± 0.5 1.5 ± 0.1 0.7 ± 0.05 0.2 ± 0.03 0.2 ± 0.009 Amies 52.3 ± 9.6 30.3 ± 1.4 34.2 ± 4.0 0.2 ± 0.002 2.1 ± 0.1 0.1 ± 0.03 0.005 ± 0.001 Stuart 44.4 ± 9.8 49.0 ± 10.2 34.5 ± 5.2 46.6 ± 11.8 8.0 ± 1.4 0.04 ± 0.006 >0.001 Dry 4.3 ± 0.5 0.6 ± 0.06 0.009 ± 0.002 0.0 0.0 − − 45°C PBST 0.0 0.0 − − − − − Amies 1.3 ± 0.1 0.0 − − − − − Stuart 1.1 ± 0.1 >0.001 0.0 0.0 − − − Dry 0.0 0.0 − − − − − VEE −70°C PBST 0.0 0.0 0.0 UTM 5.7 ± 1.1 5.48 ± 3.0 4.28 ± 0.23 M4RT 4.7 ± 0.8 10.9 ± 3.9 14.6 ± 2.8 Dry 0.13 ± 0.1 0.05 ± 0.001 0.09 ± 0.03 4°C PBST 0.0 0.0 0.0 UTM 9.1 ± 1.3 0.8 ± 0.2 5.82 ± 2.4 M4RT 4.3 ±0.4 5.75 ± 0.23 9.45 ± 0.6 Dry 0.0 0.0 0.0 30°C PBST 0.0 0.0 0.0 UTM 11.6 ± 9.9 0.0 0.0 M4RT 0.0 0.0 0.0 Dry 0.0 0.0 0.0
