Research Article

Suitability of Commercial Transport Media for Biological Pathogens under Nonideal Conditions

Table 3

ECL immunodetection of ricin toxin. A four plus system was used to indicate the number of tests that were positive according to ECL immunodetection. Duplicate swabs were extracted at each time point and set up into duplicate detection assays. Thus there is a total of four luminescent readings by which to assess whether a sample is positive (+) or negative (−) for ricin detection. Extraction and processing error on day 28 is indicated by NT (no data taken).

Biothreat agentStorage temp.Storage MediaDays in storage
13714212860

Ricin high spike−70°CWater++++++++++++++++++++++++++++
PTM++++++++++++++++++++++++++++
Dry++++++++++++++++++++++++++++
PBST++++++++++++++++++++++++++++
4°CWater++++++++++++++++++++++++++++
PTM++++++++++++++++++++++++++++
Dry++++++++++++++++++++++++++++
25°CWater++++++++++++++++++++++++++++
PTM++++++++++++++++++++++++++++
Dry++++++++++++++++++++++++++++
45°CWater++++++++++++++++++++++++++++
PTM++++++++++++++++++++++++++++
Dry+++++++++++++++++++

Ricin low spike−70°CWater++++++++++++++++++++++++++++
PTM++++++++++++++++++++++++++++
Dry++++++++++++++++++++++++++++
4°CWater++++++++++++++++++++++++++++
PTM++++++++++++++++++++++++++++
Dry++++++++++++++++
25°CWater+++++++++++++++++
PTM++++++++++++++
DryNT++
45°CWaterNT
PTMNT++++
DryNT