Direct Quantitative Detection and Identification of Lactococcal Bacteriophages from Milk and Whey by Real-Time PCR: Application for the Detection of Lactococcal Bacteriophages in Goat's Raw Milk Whey in France

Table 4

Results of real-time PCR tested with different bacteriophages.

Bacteriophages	Titration	qPCR result
		c2 primers	936 primers	P335 primers
c2 phage group
c2	1,5⋅10^5 PFU/mL	10^5 PFU/mL	No detection	No detection
p2	5,6⋅10^6 PFU/mL	6⋅10^6 PFU/mL	No detection	No detection
bIL67	2⋅10^5 PFU/mL	1,58⋅10^5 PFU/mL	No detection	No detection
P335 phage group
P335	3,4⋅10^5 PFU/mL	No detection	No detection	3⋅10^5 PFU/mL
P270	4,4⋅10^6 PFU/mL	No detection	No detection	4,5⋅10^6 PFU/mL
sk1	6,7⋅10^5 PFU/mL	No detection	No detection	7⋅10^5 PFU/mL
936 phage group
936	2,5⋅10^6 PFU/mL	No detection	3,12⋅10^6 PFU/mL	No detection
P008	5,2⋅10^5 PFU/mL	No detection	5,34⋅10^5 PFU/mL	No detection
bIL170	3,7⋅10^5 PFU/mL	No detection	3,52⋅10^5 PFU/mL	No detection