The Sulfate-Rich and Extreme Saline Sediment of the Ephemeral Tirez Lagoon: A Biotope for Acetoclastic Sulfate-Reducing Bacteria and Hydrogenotrophic Methanogenic Archaea
Table 1
The amino acid composition and G + C content of Tirez McrA and AprA sequences, their halophilic and nonhalophilic homologous counterparts, metagenomes, and reference strains.
Acid Asx + Glx
Acid − Basic6 (Asx + Glx) − (Arg + Lys)
Acid : Basic7 (Asp + Glu) : (His + Arg + Lys)
Lys
Asp : Lys
Arg
G + C % in total sequence8
G + C % in third codon position8
AprA
Tirez1
15.90 ± 2.24
2.87 ± 2.54
0.62 ± 0.13
6.90 ± 1.40
0.35 ± 0.22
6.14 ± 0.90
57.60 ± 4.56
76.00 ± 9.99
Halophilic species2
14.90 ± 2.74
2.70 ± 3.02
0.59 ± 0.18
6.20 ± 0.90
0.30 ± 0.09
6.02 ± 0.59
55.70 ± 7.56
66.50 ± 20.70
Nonhalophilic species2
15.00 ± 1.95
2.35 ± 2.13
0.62 ± 0.12
6.30 ± 1.10
0.35 ± 0.11
6.34 ± 0.68
55.00 ± 6.94
65.50 ± 19.02
mcrA
Tirez1
22.07± 0.23
16.62 ± 0.24
1.50 ± 0.00
3.38 ± 0.62
2.04 ± 0.37
2.07 ± 0.40
47.20 ± 3.40
46.10 ± 11.70
Halophilic species3
21.09 ± 0.52
15.99 ± 0.76
1.67 ± 0.10
3.54 ± 0.29
2.08 ± 1.83
1.56 ± 0.00
51.50 ± 5.20
57.00 ± 12.60
Nonhalophilic species3, 4
21.75 ± 1.22
16.44 ± 1.63
1.75 ± 0.35
3.65 ± 0.99
2.08 ± 0.80
1.67 ± 0.3
50.30 ±7.20
56.30 ± 21.30
Metagenomic7
Dead Sea
n.d.
n.d.
1.46
n.d.
n.d.
n.d.
62–67
n.d.
Reference strains5
Halobacterium salinarum
31.80
25.36
n.d.
2.34 ± 0.04
n.d.
4.10 ± 0.12
65.7
n.d.
Halomonas elongata
25.98
17.56
n.d.
3.7
n.d.
5.25
n.d.
n.d.
Escherichia coli
26.04
15.85
n.d.
6.03 ± 0.14
n.d.
4.16 ± 0.02
50.3–50.9
n.d.
1Average composition from amino acid sequences derived from this study. 2Average composition from amino acid sequences listed in supplementary material Table S1. 3Average composition from amino acid sequences listed in supplementary material Table S2. 4Thermophilic species were not included. 5Amino acid composition of the bulk protein content in type species cultures [57]. 6PAB: amino acid proportions according to [57]. 7AB: amino proportions according to Rhodes et al.[21]. 8GC content percentage is calculated as GC% = (G + C/G + C + A + T) * 100.
