Molecular Detection of Persistent Francisella tularensis Subspecies holarctica in Natural Waters

Figure 3

Multiple alignment of lpnA sequences obtained from Ljusdal and Örebro with previously published sequences of Francisella species and subspecies and Francisella-like endosymbionts (FLE). The nucleotide positions 620 to 695 refer to F. t. holarctica LVS (M32059). Reference sequences from GenBank: F. t. holarctica LVS (M32059), F. t. tularensis strain WY96-3418 (CP000608), F. t. tularensis strain Schu S4 (NC_006570), F. t. mediasiatica strain FSC147 (NC_010677), F. t. novicida strain U112 (CP000439), Dermacentor variabilis FLE (AY375420), Amblyomma maculatum FLE (AY375422), Dermacentor andersonii FLE (AY375413), Dermacentor hunteri FLE (AY375417), Dermacentor variabilis FLE (AY375421), F. philomiragia (AY243030) and F. piscicida (DQ825765).