Polymorphic Amplified Typing Sequences and Pulsed-Field Gel Electrophoresis Yield Comparable Results in the Strain Typing of a Diverse Set of Bovine Escherichia coli O157:H7 Isolates

Table 2

XbaI -based PATS profiles of the 25 bovine O157 isolates. AvrII-based PATS profiles of the 25 bovine O157 isolates.

(a)   

PATS type^a	PCR amplification and restriction digestion patterns of amplicons obtained using 8 PATS primer pairs^b								Isolates^c
	IK8	IK19	IK25	IK114	IK118	IK123	IKB3	IKB5
1	2	2	0	2	2	2	2	2	168, 908, 928,
2	0	2	0	2	2	2	2	2	977
3	2	2	0	2	2	2	0	2	5, 757, 806, 817, 1015, 1492
4	0	2	0	2	2	2	0	2	807
5	2	2	0	2	2	2	0	0	268, 827, 935
6	2	2	0	2	2	0	0	0	1041, 5671, 5974
7	2	2	0	0	2	2	2	0	1273
8	2	2	0	0	2	2	0	0	214, 528, 1016, 1286, 1328
9	2	2	0	2	0	2	0	0	425
10	0	2	0	0	2	0	0	0	961

(b)   

PATS type^a	PCR amplification and restriction digestion patterns of amplicons obtained using 7 PATS primer pairs^d							Isolates^c
	IKNR3	IKNR7	IKNR10	IKNR12	IKNR16	IKNR27	IKNR33
1	2	2	2	2	2	2	2	5, 168, 817, 908, 928, 977, 1015, 1492
2	2	2	2	0	2	2	2	268, 757, 806, 807
3	2	2	2	2	1	2	2	1041
4	0	2	2	2	1	2	2	827, 5671, 5974
5	1	2	2	2	1	2	0	214, 528, 961, 1016, 1273, 1286, 1328
6	2	1	1	2	1	2	2	425, 935

^aPATS types are designated arbitrarily with different numbers.
^bPrefixes of each PATS primer pair A/B are indicated. 0: no amplicon; 2: amplicon with one XbaI site.
^cBovine E. coli O157:H7 isolates from different locations that fell within a given PATS type.
^dPrefixes of each PATS primer pair A/B are indicated. 0: no amplicon; 1: amplicon without AvrII site; 2: amplicon with one AvrII site.