Electron Beam Irradiation Dose Dependently Damages the Bacillus Spore Coat and Spore Membrane

Table 2

Fluorescence intensity of EB-irradiated Bacillus spores after SYTO-9 and propidium iodide (PI) uptake.^a

EB dose (kGy)	SYTO-9± SD	PI ± SD	SYTO-9/PI
0	9441 ± 4280	9365 ± 1854	1.01
5.3	10293 ± 3203	9286 ± 1487	1.11
10.4	5328 ± 3163	8144 ± 2120	0.65^†
16.0^b	3703 ± 1656	9144 ± 1347	0.41^†
21.7^b	4190 ± 503	9881 ± 946	0.42^†

^aMean ± standard deviation for spores identified in Q2 of Figure 4
^bLess than 50,000 spores available to evaluate
^†𝑃≤0.001 by Tukey-Kramer test, as compared to 0 dose (unirradiated control)