Protein Glycosylation in Aspergillus fumigatus Is Essential for Cell Wall Synthesis and Serves as a Promising Model of Multicellular Eukaryotic Development
Figure 3
Comparison of N-glycans in yeast, A. fumigates, and mammalian cells. After processing by two ER α-glucosidases, the N-glycan is further processed by the action of various α1,2-mannosidases. In S. cerevisiae, a specific ER α1,2-mannosidase converts Man9GlcNAc2 into Man8GlcNAc2, which is elongated in the Golgi to form an outer chain containing up to 200 residues of mannose. In A. fumigatus, the N-glycans on mature glycoprotein are Man6GlcNAc2. In mammalian cells, Man9GlcNAc2 is converted to Man5GlcNAc2 by the action of ER and Golgi α-mannosidases, which is the precursor for complex, hybrid, and high-mannose N-glycans.
