Differential Adaptations of Methicillin-Resistant Staphylococcus aureus to Serial In Vitro Passage in Daptomycin: Evolution of Daptomycin Resistance and Role of Membrane Carotenoid Content and Fluidity

Table 1

List of strains and MICs.

Strains	Length of serial passage (days)	Gene mutation(s) ^b	DAP (𝜇g/mL)	VAN (𝜇g/mL)	OX (𝜇g/mL)
Parental CB1118 (MW2)		—	1	2	24
CB2206	1	acetate coA ligase ^a	1.5	2	24
CB2207	6	acetate coA ligase; 𝑚𝑝𝑟𝐹T345A	3	3	2
CB2208	9	acetate coA ligase; 𝑚𝑝𝑟𝐹T345A; 𝑦𝑦𝑐𝐺R263C	4	4	2
CB2209	13	acetate coA ligase; 𝑚𝑝𝑟𝐹T345A; 𝑦𝑦𝑐𝐺R263C  𝑟𝑝𝑜𝐵A1086V	8	6	2
CB2210	20	acetate coA ligase; 𝑚𝑝𝑟𝐹T345A; 𝑦𝑦𝑐𝐺R263C; 𝑟𝑝𝑜𝐵A1086V; 𝑟𝑝𝑜𝐶Q961K	16	6	2

^aNoncoding region of coenzyme A.
^bThe data have been previously published (s).