Expression of Four Methionine Sulfoxide Reductases in Staphylococcus aureus
Figure 4
Expression of the msr(A1/B), msrA2, and msrA3 loci in S. aureus SH1000 in the presence of different oxidizing chemical agents. Cultures grown to OD600 of 0.3 at 37°C were treated separately with the following stresses for 1 h: diamide (5 mM) (2), N-ethylmaleimide (NEM) (0.05 mM) (3), methyl viologen (MV) (20 mM) (4), menadione (MD) (0.05 mM) (5), cumene peroxide (CuOOH) (0.0125%) (6), and sodium nitroprusside (SNP) (5 mM) (7). β-galactosidase activity (lighter bar) and growth (OD600) (darker bar) were subsequently determined. β-galactosidase activity and growth of cells in TSB control are represented in bars 1. Values indicate averages of data from three independent experiments ± standard deviation (SD).
