Expression of Four Methionine Sulfoxide Reductases in Staphylococcus aureus

Table 1

Bacterial strains and plasmids used in this study.

Strain or plasmid	Characteristics	Reference
Strains
S. aureus SH1000	S. aureus strain 8325-4 with functional RsbU	[41]
S. aureus RN4220	A restriction minus derivative of S. aureus 8325-4	[42]
VKS1009	SH1000 with msrA1/B-lacZ integration (Erm^r)	This study
VKS1010	SH1000 with msrA2-lacZ integration (Erm^r)	This study
VKS1011	SH1000 with msrA3-lacZ integration (Erm^r)	This study
E. coli JM109	recA1 supE44 endA1 hsdR17 gyrA96 relA1thi Δ(lac-proAB) F′(traD36proAB^+lacl^qΔM15)	[43]
Plasmids
pGEMT	Cloning vector for E. coli (Amp^r)	Promega
pAZ106	lacZ fusion vector (Amp^r, E. coli; Erm^r, S. aureus)	[27]
pGEMT-msrA2P	pGEMT containing the msrA2 promoter fragment	This study
pGEMT-msrA3P	pGEMT containing the msrA3 promoter fragment	This study
pAZ-msrA2P	pAZ106 containing msrA2 promoter-lacZ fusion	This study
pAZ-msrA3P	pAZ106 containing msrA3 promoter-lacZ fusion	This study

Erm^r: erythromycin resistant, Amp^r: ampicillin resistant.