Application of Bioluminescence Imaging for In Vivo Monitoring of Fungal Infections

Figure 2

Visualisation of bioluminescence from A. fumigatus colonies. The A. fumigatus wild-type strain CBS144.89 and the bioluminescent strains C3 and 2/7/1 are shown. Strains were grown for 48 h in the presence of 1 mM D-luciferin on glucose minimal medium. The upper lane shows a daylight photograph, the bottom line bioluminescence images of the colonies acquired by a medium sensitive Versa Doc luminescence imaging system. Strain C3 carries four ectopic integrations of the P. pyralis luciferase gene codon adapted for expression in mammalian cells. Only a moderate light emission is detected. Strain 2/7/1 carries two integrations of the P. pyralis gene codon optimised for expression in A. fumigatus. Light emission is strongly enhanced.