Antioxidant Functions of Nitric Oxide Synthase in a Methicillin Sensitive Staphylococcus aureus

Figure 1

PCR verification of a mutation in the SaNOS gene in S. aureus. Primers P7 (5′-ATACAGAAGAAGAACTTATTTATGG-3′) and P8 (5′- CACCTCTACTAACTTAATGATGG-3′) were used in the PCR that allowed amplification of a 963 bp product (lane 1) when genomic DNA from wild-type S. aureus strain SH1000 was used. These primers amplified a ~2.4 kb fragment when genomic DNA from the SaNOS mutant of S. aureus strains SH1000 was used as template (lanes 2). Lane 3: PCR product when genomic DNA from the SaNOS mutants of S. aureus strains SH1000 complemented in trans with SaNOS was used as template. The larger PCR product is not seen because of complementation with wild-type SaNOS gene on a high copy plasmid pCU1. Lane M: DNA ladder.