Research Article

Effect of Citrus Byproducts on Survival of O157:H7 and Non-O157 Escherichia coli Serogroups within In Vitro Bovine Ruminal Microbial Fermentations

Table 1

Least squares means for growth of  STEC O26:H11, O103:H8, O111:H8, O145:H28, O157:H7, and O157:H7 within bovine mixed rumen microorganisms fluid medium, supplemented with 0%, 5%, 10%, and 20% powdered citrus by-products (CBPs).

CBPTime (h)E. coli serotype (Log10 CFU/mL)
O26:H11O103:H8O111:H8O145:H28O157:H7O157:H7

0 h7.55 7.11 6.93 7.55 6.03v 6.46
2 h7.68 7.05 6.70 7.58 6.91w 7.00
0%4 h7.60 6.81 6.92 8.06 6.91w 7.19
6 h7.73 6.65 7.07 7.83 7.24w 6.98
0.18−0.0460.140.281.210.52

0 h7.40 7.34y 7.31v 7.57 6.73 6.63
2 h7.47 6.86yz 7.06v 7.64 6.65 6.41
5%4 h7.43 6.95yz 5.85w 7.80 6.77 7.09
6 h7.59 6.55z 7.22v 7.80 7.02 6.74
0.23−0.590.61−0.10.15−2.63

0 h7.43 7.28 6.64 7.736.83 6.82vw
2 h7.43 6.90 7.33 7.567.22 6.18w
10%4 h7.36 6.99 7.18 7.646.96 7.13v
6 h7.66 6.69 7.25 7.636.98 4.19x
0.07−0.47−0.320.06−0.03−5.04

0 h7.12v 7.13 7.02 7.607.03 7.03v
20%2 h7.54v 7.33 6.78 7.526.94 6.53v
4 h 5.96w 7.07 6.65 7.44 7.30 7.34v
6 h7.19v 6.66 6.70 7.66 7.00 1.99w

aChange in concentration between 0 h and 6 h.
v,w,xLsmeans within a column, within a treatment, without a common subscript are different if .
y,zLsmeans within a column, within a treatment, without a common subscript tend to be different if .