Research Article

Effect of Citrus Byproducts on Survival of O157:H7 and Non-O157 Escherichia coli Serogroups within In Vitro Bovine Ruminal Microbial Fermentations

Table 2

Least squares means for growth of STEC O26:H11, O103:H8, O111:H8, O145:H28, O157:H7, and O157:H7 within bovine mixed rumen microorganisms fluid medium, supplemented with 0%, 5%, 10%, and 20% pelleted citrus by-products (CBPs).

CBPTime (h)E. coli serotype (Log10 CFU/mL)
O26:H11O103:H8O111:H8O145:H28O157:H7O157:H7

0 h 9.27x7.01x8.47x9.618.48 6.46
2 h 8.08y7.01x 9.01xy9.488.24 6.27
0%4 h 9.45x8.64y8.43x9.678.79 6.26
6 h 9.64x8.77y9.48y9.509.01 6.51
Δa0.371.761.01−0.110.530.05

0 h 9.27xy8.21 8.40xy9.51 9.23x 6.07
2 h 8.71x8.268.93x9.60 7.94y 5.92
5%4 h 7.29z7.937.98y9.59 7.96y 6.23
6 h 8.71y8.288.77x9.69 8.93x 5.97
−0.560.070.370.18−0.3−0.1

0 h 9.07xz8.76 8.76xy8.98 8.73xy 6.83
2 h 8.43xy8.50 9.03xy9.01 8.73xy 6.94
10%4 h 7.90y8.37 8.66x9.55 8.40xy 6.64
6 h 8.59z8.07 9.73y9.71 9.20y 6.68
−0.48−0.690.970.730.47−0.15

0 h 9.47x 8.52x9.03 9.29x 8.00x 6.78
2 h 8.98x 8.51x8.92 9.79xy 8.00y 6.76
20%4 h 7.69y 8.57x9.09 8.95y 8.71xy 6.76
6 h 9.54x 7.43y9.46 9.48xy 9.05y 7.13
−0.07−1.09−0.430.191.050.35

aChange in concentration between 0 h and 6 h.
x,y,zLsmeans within a column, within a treatment, without a common subscript are different if .