Research Article

Bacteriophage-Derived Peptidase Eliminates and Prevents Staphylococcal Biofilms

Figure 4

  biofilm prevention assay: viable plate count. Concentrations of enzyme ranging from 7.8−125 μg mL−1 were incubated with S. aureus DPC5246 in TSBg at 37°C for 24 h in a 96 well peg plate. The pegs were washed after incubation and subsequently any cells remaining adhered to the pegs were removed by centrifugation at 800 rpm for 20 mins. Assays were carried out in triplicate and data was expressed as the mean ± SE. The values indicated above the bars are the mean CFU/mL after treatment.
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