DGGE Identification of Microorganisms Associated with Borrelia burgdorferi Sensu Lato- or Anaplasma phagocytophilum-Infected Ixodes ricinus Ticks from Northwest Norway
Table 2
Identification of bacteria associated with ticks obtained by sequence analysis of reamplified DGGE bands.
Species numberb
Closest related sequencea
Percent similarity
GenBank ID
B. burgdorferi sensu lato-infected ticks
A. phagocytophilum-infected ticks
Ticks not infected by B. burgdorferi sensu lato or A. phagocytophilum
G1 ( )c
%
G2 ( )
%
G3 ( )
%
1
Candidatus Midichloria mitochondrii
100%
CP002130.0
16 (40)
40
10 (40)
25
10 (40)
25
2
Pseudomonas spp.
100%
JQ598792.1
25 (40)
62,5
31 (40)
77,5
40 (40)
100
3
Pseudomonas spp.
100%
JN630834
2 (40)
5
2 (40)
5
8 (40)
20
4
Pseudomonas fluorescens
100%
JX131014.1
5 (40)
12,5
12 (40)
30
9 (40)
22,5
5
100%
EF428995.1
4 (40)
10
10 (40)
25
0 (40)
0
6
Pseudomonas putida
99%
HQ18860l.1
7 (40)
17,5
0 (40)
0
2 (40)
5
7
Spiroplasma spp.
100%
AJ132412.1
2 (40)
5
0 (40)
0
0 (40)
0
8
Roseomonas spp.
100%
DQ532254
5 (40)
12,5
3 (40)
7,5
0 (40)
0
9
Sphingomonas spp.
100%
NR044341
0 (40)
0
12 (40)
30
5 (40)
12,5
10
Sphingomonas spp.
100%
JQ660272.1
15 (40)
37,5
11 (40)
27,5
0 (40)
0
11
Sphingomonas spp.
<95%
16 (40)
40
0 (40)
0
2 (40)
5
12
Methylovirgula spp.
99%
FM252035.1
7 (40)
17,5
0 (40)
0
0 (40)
0
13
Methylobacterium spp.
100%
JQ617889.1
0 (40)
0
0 (40)
0
4 (40)
10
14
<95%
5 (40)
12,5
0 (40)
0
0 (40)
0
15
Burkholderia spp.
100%
FN298915.1
0 (40)
0
0 (40)
0
3 (40)
7,5
16
Erwinia billingiae
100%
FP236843.1
0 (40)
0
0 (40)
0
2 (40)
5
17
Erwinia billingiae
100%
AM117487.1
9 (40)
22,5
0 (40)
0
0 (40)
0
18
Raoultella spp.
100%
AY292873
2 (40)
5
0 (40)
0
0 (40)
0
19
Enterobacter spp.
100%
JN850605.1
6 (40)
15
0 (40)
0
0 (40)
0
20
Enterobacteriaceae
99%
JX162048.1
0 (40)
0
2 (40)
5
0 (40)
0
21
Stenotrophomonas rhizophila
100%
JX005877.1
0 (40)
0
1 (40)
2,5
0 (40)
0
22
Williamsia spp.
100%
FN550136.1
0 (40)
0
1 (40)
2,5
2 (40)
5
23
Uncultured Mycobacterium spp.
100%
GQ203456.1
0 (40)
0
1 (40)
2,5
1 (40)
2,5
24
Bacillus spp.
100%
NR042286.1
6 (40)
15
0 (40)
0
0 (40)
0
25
Bacillus spp.
100%
EU647705
14 (40)
35
0 (40)
0 (40)
0
26
Mycobacterium
<95%
0 (40)
0
0 (40)
0
3 (40)
7,5
27
Beijerinckiaceae
<95%
0 (40)
0
0 (40)
0
1 (40)
2,5
28
Luteibacter rhizovicinus
100%
AB627008
0 (40)
0
2 (40)
5
0 (40)
0
29
I. ricinus 18S rDNA
100%
The source of each sequence was identified using BLAST to compare sequences from the DGGE analysis to sequences of known bacterial species in the GenBank database [24]. Sequences that exhibited less than 95% identification by BLAST search were classified by the RDP classifier [25].
bSpecies number corresponds with the numbering of bands on the DGGE gel as shown in Figures 1(a), 1(b), 1(c), and 2.
c : number of positive ticks in this group, (): total number of ticks analysed in this group.
