Effect of Noradrenaline on the Virulence Properties of Campylobacter Species
Figure 5
Effect of Campylobacter on integrity of T84 monolayers. Monolayers were fixed and stained for tight junction protein occludin after 48-hour infection with Campylobacter or Campylobacter with NA and analysed by confocal microscopy. Monolayers exposed to C. jejuni 81116, C. jejuni M1, C. coli, and C. fetus fetus in the absence of noradrenaline shown as control in (b)–(e) (A) (the first figure). Cell monolayers co-infected with Campylobacter in the presence of noradrenaline are shown as treated group in (b)–(e) (B) (the second figure). Ten fields of view were visualised using confocal microscopy with a Hamamatsu camera and digitised using Leica Q fluoro software and analysed by quantitative immunofluorescence using Macros on image J software. The average area of percentage area of occludin staining was measured in ten views and compared by an unpaired test ((a)–(e) (C)). Results shown are from a single experiment; *represents value <0.05.
