Molecular Typing of Methicillin Resistant Staphylococcus aureus Clinical Isolates on the Basis of Protein A and Coagulase Gene Polymorphisms

Table 1

Combined coa gene typing and HaeIII RFLP patterns of the 75 isolated MRSA strains.

coa band types (3)	coa gene subtypes (10)	RFLP pattern (12)	Size of PCR product (approximate bp)	Size of HaeIII fragments (approximate bp)	Total isolates number (%)	Isolate serial number
Co1  (1 band); 16/75 (21.3%)	Co1a	C1 (3 bands)	810	2 (162), 486	3 (4)	7, 12, 13
	Co1b	A1 (uncut)	891	891	2 (2.7)	43, 49
		B (2 bands)	891	324, 567	3 (4)	56, 62, 68
		C2 (3 bands)	891	2 (162), 567	7 (9)	51–55, 57, 58
	Co1c	A2 (uncut)	1215	1215	1 (1.3)	46
Co2  (2 bands); 54/75 (72%)	Co2a	D1 (4 bands)	405, 810	2 (162), 405, 486	44 (58.7)	1–6, 9–11, 16, 18–20, 22–28, 30–35, 37–42, 45, 47, 50, 60, 61, 65, 66, 71–75
	Co2b	D2 (4 bands)	405, 729	81, 162, 405, 486	1 (1.3)	17
	Co2c	D3 (4 bands)	405, 891	2 (162), 405, 567	7 (9)	44, 48, 59, 63, 67, 69, 70
	Co2d	C3 (3 bands)	81, 810	81, 2 (405)	1 (1.3)	14
	Co2e	E1 (5 bands)	405, 972	81, 162, 324, 405, 486	1 (1.3)	36
Co3 (3 bands); 5/75 (6.7%)	Co3a	E2 (5 bands)	81, 405, 810	81, 2 (162), 405, 486	4 (5.3)	8, 15, 21, 29
	Co3b	A3 (uncut)	243, 486, 1215	243, 486, 1215	1 (1.3)	64