The TIR Domain Containing Locus of Enterococcus faecalis Is Predominant among Urinary Tract Infection Isolates and Downregulates Host Inflammatory Response
Figure 3
TcpF is constitutively transcribed in E. faecalis Symbioflor 1. (a) Full length mRNA transcript of tcpF in E. faecalis Symbioflor 1. Reverse transcription was performed with primer E.f.seq.2. PCR primers E.f.seq. 8/15: 457 bp, primers E.f.seq. 8/14: 640 bp, primers E.f.seq. 8/17a: 671 bp, and primers E.f.seq. 8/1: 680 bp. (b) TcpF mRNA is transcribed upon growth in BHI medium and upon infection on CaCo cells. PCR primers E.f.seq. 7/6: 300 bp. Reverse transcriptase minus (RT-) reactions served as negative control.
