The TIR Domain Containing Locus of Enterococcus faecalis Is Predominant among Urinary Tract Infection Isolates and Downregulates Host Inflammatory Response

Figure 5

Intracellular expression of TcpF impairs TLR2 dependent NF𝜅B activation. HEK293 cells were transiently transfected with hTLR2 (50 ng), pRK5, reporter plasmids pELAM-Luc and phRL-TK, and increasing amounts of TcpF (a) or TcpC1 (b) expressing plasmids at ratios of 1 : 1, 1 : 2, 1 : 5, 1 : 10, and 1 : 31. Cells were stimulated with 0.2 μM PAM3 and incubated for 5 h. Nonstimulated and TLR2 deficient cells (TLR2-) served as controls. Error bars represent s.d. (𝑛=3). RLU: relative luciferase unit.

(a)

(b)