Effect of Trehalose and Trehalose Transport on the Tolerance of Clostridium perfringens to Environmental Stress in a Wild Type Strain and Its Fluoroquinolone-Resistant Mutant

Table 1

Primers designed for the amplification of trehalose operon genes in C. perfringens along with the upstream regions.

Name	Gene	Sequence	Location	Size
CPF_0539F	Sigma-70 factor	TGAGTTCCAAAATAGACAAGAT	640794–640815	22-mer
CPF_0539R	Sigma-70 factor	CCCCATAAGCCAAGGTG	642208–642224	17-mer
CPF_0540F	Hypothetical protein	AATGGGGTAGGAATAACTTTGTCA	642326–642349	24-mer
CPF_0540R	Hypothetical protein	CTTTTCCGCCTATGTACTCTGTTA	643493–643516	24-mer
1985F	CPF_0540 upstream	ACAAGCTTTAGGTGAGGTTT	641985–642004	20-mer
tre373F	treB	TGATATGAATAAGGAGGGTGTTGG	642793–642816	24-mer
tre1027F	treB	TATGGAGGGAAAAAGATGAGTAAG	643447–643470	24-mer
tre2172R	treB	TGCTGGTATTGATATTTGTTTTTC	644569–644592	24-mer
tre1920F	treB	TATAGTGGCAGGCTTAACATCATC	644340–644363	24-mer
tre3128R	treC	CTCTCCTAGCTCCTCAACATACTC	645525–645548	24-mer
tre3090F	treC	GGAGGGAATGCTTGGGAGTA	645510–645529	20-mer
tre3777R	treC	AACCTGGATTTGTCATTC	646180–646197	18-mer
tre3688F	treC	CTTCAAAAATGCTAGCGACTTCAA	646108–646131	24-mer
tre4667R	treC-treR	TGCTTCAAAAATTCCACTATCTA	647065–647087	23-mer
tre4406F	treR	AAATTAAGAAGGGGTTGTAT	646826–646845	20-mer
tre5283R	treR	ACTTAAATTTATCTGGTCTATGTC	647680–647703	24-mer
treRR	treR	CTTTAATAGCTGGTGATAGTT	648367–648387	21-mer