Microbiological Quality Assessment by PCR and Its Antibiotic Susceptibility in Mangrove Crabs (Ucides cordatus) from Guanabara Bay, Rio de Janeiro, Brazil

Table 2

Oligonucleotide sequences used for the identification of the Vibrio genus and serotypes.

Serotypes	Oligonucleotides	Gene	Sequences (5′-3′)	Fragment size (bp)
Vibrio spp.	V.16S-700F	16S	CGG TGA AAT GCG TAG AGA T	663
	V.16S1325R		TTA CTA GCG ATT CCG AGT TC
V. cholerae	Vc.sodB-F	sodB	AAG ACC TCA ACT GGC GGT A	248
	Vc.sodB-R		GAA GTG TTA GTG ATC GCC AGA GT
V. mimicus	Vm.sodB-F	sodB	CAT TCG GTT CTT TCG CTG AT	121
	Vm.sodB-R2		GAA GTG TTA GTG ATT GCT AGA GAT
V. parahaemolyticus	Vp.flaE-79F	flaE	GCA GCT GAT CAA AAC GTT GAG T	897
	Vp.flae-934R		ATT ATC GAT CGT GCC ACT CAC
V. vulnificus	Vv.hsp-326F	hsp	GTC TTA AAG CGG TTG CTG C	410
	Vv.hsp-697R		CGC TTC AAG TGC TGG TAG AAG