Research Article

Gene Expression and Molecular Characterization of a Xylanase from Chicken Cecum Metagenome

Table 1

Substrate specificity of the purified recombinant

Relative activity

Beechwood xylan100
Oat-spelt xylan94
Carboxymethyl cellulose0
α-Cellulose0
Starch0
Barley -glucan0
4-nitrophenyl--D-xylopyranoside55
4-nitrophenyl--D-cellobioside0
4-nitrophenyl-α-D-galactopyranoside0

test concentration for polysaccharide substrates was 1% (w/v) while that for the synthetic chromogenic substrates was 4 mg/mL. activity towards beechwood xylan which was the highest activity was defined as 100%. All the values are means of three replications.