Growth on Alpha-Ketoglutarate Increases Oxidative Stress Resistance in the Yeast Saccharomyces cerevisiae

Figure 2

Effect of exposure to 10 mM H₂O₂ for 30 min on cell survival in S. cerevisiae YPH250 strain and its isogenic derivatives grown in the absence or presence of 10 mM AKG. (a) YPH250 cells (wild type) grown with AKG different concentrations; (b) mutant cells grown without or with 10 mM AKG; (c) Δcta1Δctt1 cells grown without or with AKG and were treated with only 10 mM H₂O₂ denoted as “control” and “AKG (growth),” respectively; “AKG (incubation),” Δcta1Δctt1 cells grown without AKG were treated with 10 mM H₂O₂ in combination with 10 mM AKG. Data are means ± SEM, . different from respective control values with using Dunnett’s test (a) or Student’s t-test (b, c).

(a)

(b)

(c)