Growth on Alpha-Ketoglutarate Increases Oxidative Stress Resistance in the Yeast Saccharomyces cerevisiae
Table 1
Growth of different S. cerevisiae strains measured by absorbance at 620 nm () at certain time periods.
Growth conditions
Time, h
Strain
YPH250
Δcta1Δctt1
Δsod1
Δsod1Δsod2
Δgsh1
Δyap1
Control (without AKG)
0
0.095 ± 0.015
0.085 ± 0.010
0.105 ± 0.015
0.087 ± 0.015
0.107 ± 0.015
0.88 ± 0.012
6
0.117 ± 0.020
0.099 ± 0.011
0.127 ± 0.023
0.097 ± 0.011
0.111 ± 0.021
0.139 ± 0.030
15
1.012 ± 0.035
0.946 ± 0.099
1.125 ± 0.065
1.306 ± 0.134
24
1.178 ± 0.086
1.185 ± 0.058
1.014 ± 0.045
1.119 ± 0.074
1.224 ± 0.125
1.558 ± 1.155
48
1.575 ± 0.135
1.381 ± 0.125
1.505 ± 0.110
1.68 ± 0.215
10 mM AKG
0
0.098 ± 0.016
0.083 ± 0.012
0.102 ± 0.017
0.085 ± 0.011
0.101 ± 0.011
0.095 ± 0.013
6
0.105 ± 0.012
0.098 ± 0.013
0.115 ± 0.018
0.099 ± 0.011
0.115 ± 0.025
0.115 ± 0.025
15
1.075 ± 0.058
0.954 ± 0.065
1.154 ± 0.077
1.325 ± 0.145
24
1.179 ± 0.046
1.195 ± 0.095
1.121 ± 0.098
1.103 ± 0.105
1.248 ± 0.095
1.611 ± 0.173
48
1.601 ± 0.103
1.377 ± 0.141
1.550 ± 0.125
1.672 ± 0.185
Cells were cultured in YPD medium supplemented with 10 mM AKG. The starting cell concentration was about 0.3 × 106 cells/mL. Data are means ± SEM, . difference from respective values for YPH250 (wide type) strain with using Student’s t-test.