Growth on Alpha-Ketoglutarate Increases Oxidative Stress Resistance in the Yeast Saccharomyces cerevisiae

Table 1

Growth of different S. cerevisiae strains measured by absorbance at 620 nm () at certain time periods.

Growth conditions	Time, h	Strain
		YPH250	Δcta1Δctt1	Δsod1	Δsod1Δsod2	Δgsh1	Δyap1
Control (without AKG)	0	0.095 ± 0.015	0.085 ± 0.010	0.105 ± 0.015	0.087 ± 0.015	0.107 ± 0.015	0.88 ± 0.012
	6	0.117 ± 0.020	0.099 ± 0.011	0.127 ± 0.023	0.097 ± 0.011	0.111 ± 0.021	0.139 ± 0.030
	15	1.012 ± 0.035	0.946 ± 0.099			1.125 ± 0.065	1.306 ± 0.134
	24	1.178 ± 0.086	1.185 ± 0.058	1.014 ± 0.045	1.119 ± 0.074	1.224 ± 0.125	1.558 ± 1.155
	48	1.575 ± 0.135	1.381 ± 0.125			1.505 ± 0.110	1.68 ± 0.215
10 mM AKG	0	0.098 ± 0.016	0.083 ± 0.012	0.102 ± 0.017	0.085 ± 0.011	0.101 ± 0.011	0.095 ± 0.013
	6	0.105 ± 0.012	0.098 ± 0.013	0.115 ± 0.018	0.099 ± 0.011	0.115 ± 0.025	0.115 ± 0.025
	15	1.075 ± 0.058	0.954 ± 0.065			1.154 ± 0.077	1.325 ± 0.145
	24	1.179 ± 0.046	1.195 ± 0.095	1.121 ± 0.098	1.103 ± 0.105	1.248 ± 0.095	1.611 ± 0.173
	48	1.601 ± 0.103	1.377 ± 0.141			1.550 ± 0.125	1.672 ± 0.185

Cells were cultured in YPD medium supplemented with 10 mM AKG. The starting cell concentration was about 0.3 × 10⁶ cells/mL. Data are means ± SEM, . difference from respective values for YPH250 (wide type) strain with using Student’s t-test.