Phenotypic and Genotypic Characterization of Extended Spectrum Beta-Lactamase-Producing Clinical Isolates of Escherichia coli and Klebsiella pneumoniae in Two Kenyan Facilities: A National Referral and a Level Five Hospital

Table 1

Primers utilised for PCR amplification of ESBL-associated resistant genes in clinical isolates of E. coli and K. pneumoniae in this study.

Primer name	Sequence (5′-3′)	Target ESBL gene	Amplicon size (bp)
MultiTSO-T_forward	CAT​TTC​CGT​GTC​GCC​CTT​ATT​C	TEM including TEM-1 and TEM-2	800
MultiTSO-T_reverse	CGT​TCA​TCC​ATA​GTT​GCC​TGA​C
MultiTSO-S_forward	AGC​CGC​TTG​AGC​AAA​TTA​AAC	SHV-including SHV-1	713
MultiTSO-S_reverse	ATC​CCG​CAG​ATA​AAT​CAC​CAC
MultiTSO-O_forward	GGC​ACC​AGA​TTC​AAC​TTT​CAA​G	OXA-1, OXA-4 and OXA-30	564
MultiTSO-O_reverse	GAC​CCC​AAG​TTT​CCT​GTA​AGT​G
MultiCTXMGp1_forward	TTAGGA AATGTGCCGCTGCA	CTX-M-1, CTX-M-3 and CTX-M-15	688
MultiCTXMGp1-2_reverse	CGATATCGTTGGTGGTGC CAT
MultiCTXMGp2_forward	CGTTAACGGCACGATGAC	CTX-M-2	404
MultiCTXMGp1-2_reverse	CGA​TAT​CGT​TGG​TGG​TGC​CAT
MultiCTXMGp9_forward	TCA AGCCTGCCGATCTGGT	CTX-M-9 and CTX-M-14	561
MultiCTXMGp9_reverse	TGATTCTCGCCGCTGAAG
CTX-Mg8/25_forward	AACACACAGACGCTCTAC	CTX-M-8, CTX-M-25, CTX-M-26 and CTX-M-39 to CTX-M-41	326
CTX-Mg8/25_reverse	TCG​AGC​CGG​AAG​GTG​TTA​T

Adopted from Dallenne et al. [30].