Phenotypic and Genotypic Characterization of Extended Spectrum Beta-Lactamase-Producing Clinical Isolates of Escherichia coli and Klebsiella pneumoniae in Two Kenyan Facilities: A National Referral and a Level Five Hospital
Table 8
Frequency of the E. coli and K. pneumoniae isolates expressing the ESBL genotypes.
ESBL gene
ESBL status
E. coli
K. pneumoniae
N (%)
ELVH n (%)
KNH n (%)
value
n (%)
ELVH n (%)
KNH n (%)
value
n (%)
blaTEM
Positive
4 (11.8)
46 (44.2)
0.5376
58 (42.0)
12 (100.0)
94 (81.7)
>0.9999
113 (89.0)
171 (64.5)
Negative
1 (2.9)
7 (6.8)
0 (0.0)
7 (6.1)
blaSHV
Positive
28 (82.4)
19 (18.3)
0.2721
56 (40.6)
6 (50.0)
91 (79.1)
>0.9999
105 (82.7)
161 (60.8)
Negative
3 (8.8)
6 (5.7)
0 (0.0)
8 (7.0)
blaOXA
Positive
13 (17.6)
31 (29.8)
0.3192
50 (36.2)
2 (16.7)
88 (76.5)
>0.9999
97 (76.4)
144 (54.3)
Negative
0 (0.0)
6 (5.8)
0 (0.0)
7 (6.1)
blaCTX-M-gp-1
Positive
5 (14.7)
23 (22.1)
>0.9999
28 (20.3)
4 (33.3)
86 (74.8)
>0.9999
92 (72.5)
117 (44.2)
Negative
0 (0.0)
0 (0.0)
0 (0.0)
2 (1.7)
blaCTX-M-gp-9
Positive
3 (8.8)
21 (20.2)
>0.9999
24 (17.4)
1 (8.3)
0 (0.0)
>0.9999
1 (0.8)
25 (9.4)
Negative
0 (0.0)
0 (0.0)
0 (0.0)
0 (0.0)
blaCTX-M-gp-2
Positive
7 (20.6)
9 (8.7)
0.2632
19 (13.8)
1 (8.3)
1 (0.85)
>0.9999
3 (2.4)
22 (8.3)
Negative
0 (0.0)
3 (2.8)
0 (0.0)
1 (0.85)
blaCTX-M-gp-8/25
Positive
0 (0.0)
0 (0.0)
NC
0 (0.0)
0 (0.0)
0 (0.0)
NC
0 (0.0)
0 (0.0)
Negative
0 (0.0)
0 (0.0)
0 (0.0)
0 (0.0)
Fisher’s exact test at α0.05; ESBL: Extended-spectrum β-Lactamase; n: sample size; N: total number of KNH: Kenyatta national hospital; ELVH: Embu level V hospital; n: sample size; N: total number of isolates. The values in parenthesis indicate the percentage proportion of clinical isolates expressing the ESBL-associated genotypes.
