Impairment and Differential Expression of PR3 and MPO on Peripheral Myelomonocytic Cells with Endothelial Properties in Granulomatosis with Polyangiitis
Figure 5
(a) Immunoblot analysis of eEOC plasma membrane preparations for mPR3 expression. All but one out of five GPA patients displayed significant mPR3 expression. The one individual with intense mPR3 signal was a patient that refused therapy from the beginning of the disease. (b)–(d) Laser scanning analysis of cultures eEOCs. The cells were detected by UE lectin binding (green immunofluorescence in (c)) and Dil-acLDL uptake lectin binding (red immunofluorescence in (c)). All double-positive cells were analyzed for mPR3 expression (long red in (d)). Only a minority of the cells was mPR3 positive. (e)–(i) Representative images of PR3 expression in cultured peripheral eEOCs. Cells were stained for UE lectin ((f)—green), Dil-acLDL uptake ((g)—orange), and PR3 ((h)—red). Image e merges images (e)–(h) ((e): nuclear staining with DAPI solution, magnification in (e)–(i) × 100).
