Constitutional Nephrin Deficiency in Conditionally Immortalized Human Podocytes Induced Epithelial-Mesenchymal Transition, Supported by -Catenin/NF-kappa B Activation: A Consequence of Cell Junction Impairment?
Figure 4
Effects of Ca2+ deprivation and TGF-1 on -SMA expression in wild type podocytes. The cells were exposed to the indicated treatment and processed for western blot as specified in Section 2. The antibodies used are described in the previous figures. To quantify the protein bands, we scanned the blots and performed a densitometry analysis, using the software NIH ImageJ v. 6.4 (freeware, NIH, MD, USA). The figure shows a typical experiment of at least three performed under the same conditions.
