Optical Identification of Plasmodium falciparum Malarial Byproduct for Parasite Density Estimation

Figure 2

Pseudocolor (a–c) red, green, and blue (RGB) representation of images acquired from three modes with the aid of a multispectral light-emitting diode imaging microscope. Each image consists of combined images of 660 nm (R), 525 nm (G), and 435 nm (B). (a) Reflection mode shows shades of white in most of RBCs, indicating a reduction in reflection in some RBCs with dark spots in the center. (b) Scattering mode depicts dimmer or less scattering for some RBCs. (c) Transmission mode shows some RBCs absorbing more light than others in the center of the RBCs, hence appearing as dark spots.

(a)

(b)

(c)