Research Article
Picoplankton Community Composition by CARD-FISH and Flow Cytometric Techniques: A Preliminary Study in Central Adriatic Sea Water
Table 1
Oligonucleotide probes used in the present study.
| | Probe | 5′-…………………-3′ | % FA (35°C ) | Target | Reference |
| | Eub338 | 5′-GCTGCCTCCCGTAGGAGT-3′ | 55 | Most Eubacteria | [36] | | Eub338 II | 5′-GCAGCCACCCGTAGGTGT-3′ | 55 | Planctomycetales | [37] | | Eub338 III | 5′-GCTGCCACCCGTAGGTGT-3′ | 55 | Verrucmicrobia | [37] | | Alf968 | 5′-GGTAAGGTTCTGCGCGTT-3′ | 45 | Alphaproteobacteria | [38] | | Gam42a | 5′-GCCTTCCCACATCGTTT-3′ | 55 | Gammaproteobacteria | [39] | | Bet42a (comp. for Gam42a) | 5′-GCCTTCCCACTTCGTTT-3′ | 55 | Betaproteobacteria | [39] | | CF319a | 5′-TGGTCCGTGTCTCAGTAC-3′ | 55 | Cytophaga f lavobacterium | [39] | | Sar11 | 5′-TACAGTCATTTTCTTCCCCGAC-3′ | 45 | Sar11clade | [40] | | Non338 | 5′-ACTCCTACGGGAGGCAGC-3′ | 20 | Nonsense control | [36] |
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