About this Journal Submit a Manuscript Table of Contents
International Journal of Oceanography
Volume 2013 (2013), Article ID 482451, 6 pages
http://dx.doi.org/10.1155/2013/482451
Research Article

Marine Environmental Risk Assessment of Sungai Kilim, Langkawi, Malaysia: Heavy Metal Enrichment Factors in Sediments as Assessment Indexes

Centre of Ocean Research, Conservation & Advances (ORCA), Division of Research, Industrial Linkage, Community Network & Alumni, Universiti Teknologi MARA, 02600 Arau, Perlis, Malaysia

Received 4 December 2012; Revised 12 February 2013; Accepted 13 May 2013

Academic Editor: Heinrich Hühnerfuss

Copyright © 2013 Jamil Tajam and Mohd Lias Kamal. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Concentrations of Cd, Co, Pb, and Zn in riverbed sediments from six sampling stations along the Sungai Kilim, Langkawi, Malaysia, were determined by using the Teflon Bomb Digestion. From this study, the concentrations of heavy metals in riverbed sediments were found ranging between 6.10 and 8.87 μg/g dry weight for Co, 0.03 and 0.45 μg/g dry weight for Cd, 59.8 and 74.9 μg/g dry weight for Zn, and 1.06 and 11.69 μg/g dry weight for Pb. From the observation, these areas were polluted by domestic waste, aquaculture, and tourism activities. For clarity, enrichment factor index was used to determine the level of sediment contamination in the study area. From this study, the average EF value is a bit high for Cd () followed by Zn (), Pb (), and lastly, Co (). Based on the contamination categories, Cd was categorised as moderately enriched, while the rest of the metals studied were in deficient-to-minimally enriched by the anthropogenic sources.

1. Introduction

Heavy metals are one of the most poisonous and serious groups of pollutants due to their high toxicity, abundance, and ease of accumulation from various plants and animals. It has been accepted that heavy metals can exist in the environment deriving from a variety of natural and anthropogenic sources. The phenomena of erosion, acidification, and weathering processes have brought input of these metals into the environment in a natural way. According to Idris [1], the natural occurrence of heavy metals in aquatic environments and their movement through the hydrocycle in addition to the inputs from anthropogenic activities reflect their ubiquity and complexity. Meanwhile, human activities also contribute to the existence of these metals such as industrial processes, agricultural and aquaculture activities, domestic wastes, and emission from vehicles [2].

Nowadays, these anthropogenic heavy metals contribute to the uppermost pollution to the aquatic environment, especially in the sediment. Sediment plays a major role in determining the pollution pattern of marine ecosystem [3]. According to Singh et al. [4] and Mwamburi [5], the sediments can act as both carriers and sinks for contaminants, reflecting the history of pollution while also providing a record of catchment inputs into the aquatic ecosystem. On the other hand, sediment can play a significant role as a scavenger agent for heavy metals, and an adsorptive sink in marine environment [6, 7]. Tsugonai and Yamada [8] further explained that aquatic sediment can act as scavengers of metals in the environment due to its several, sulfides, organic matter, iron and manganese oxides, and clays. Hence, the use of sediments is advantageous to assess human impacts on the marine environment.

For these reasons, the assessment of pollutants in the estuary system of Sungai Kilim, Langkawi, was carried out to perform the current sediment data assessment in order to assess its level of pollution in this area. Generally, Sungai Kilim is situated in the north-eastern region of Pulau Langkawi. The river is located at approximately 99°52′8.01′′ E and 6°25′39.75′′ N. Sungai Kilim has become one of the popular sites to be visited for its attractive ecology and features. Due to its uniqueness, ecotourism benefits towards many locals and tourists [8]. The downstream of Sungai Kilim is characterized by the high number of karst formations with high density of vegetative roots covering its surface. The middle stream is connected to two small rivers or tributaries. Beyond the upstream, urbanization conquered the banks where roads, restaurants, and tourist jetty are developed well for tourism activities.

Recently, quite a number of heavy metal assessment studies have been reported in this area. This assessment is particularly carried out in order to protect and conserve the environment of the Kilim River from further contamination by anthropogenic sources. Furthermore, the information gathered from this monitoring study may also provide an aid in the management of suitable policy for Langkawi Development Authority (LADA) to preserve the Sungai Kilim ecosystem as Kilim Karst Geoforest Park.

2. Methodology

2.1. Sample Collection and Preservation

Sample collection was conducted in December 2009 during the northeast monsoon season. Generally, Sungai Kilim, Langkawi (Figure 1) is very popular for a variety of ecotourism activities and due to this, major development has been done to improve the river’s quality for providing the best services to tourists. These include eagle feeding activities (Station 3), floating restaurants, aquaculture (Station 4), yacht anchoring area (Station 4), and also boating services (Station 6). Therefore, six stations were established along the Sungai Kilim and marked using GPS (global positioning system) (Table 1). These locations were selected based on the fact that they might have been impacted by the nearby source of contamination. The distance between each station was approximately 1 km. Riverbed sediment samples were collected using the Van Veen Grab, where afterwards, samples were placed in plastic bags which were previously immersed in 5% nitric acid for two to three days to prevent sample contamination. The sediment samples were then preserved in the ice box at 4°C to maintain the original condition of the samples. At the laboratory, samples were dried in the oven at 105°C for 24 hours. For heavy-metal analysis, it was ensured that the samples had been completely dried before grinding the samples with mortar and pestle and sieved under 63 μm size. Precautions in preventing sample contamination were given priority. Samples were then stored in labelled plastic vials and kept in the drying cabinet until lab analysis.

tab1
Table 1: The coordinates for each sampling station.
fig1
Figure 1: Sampling area.
2.2. Sample Digestion

In this study, the digestion and analytical procedures were adopted and applied from those of Kamaruzzaman [9], Jamil [10], and Trimm et al. [11] with little modifications. For this analysis, 0.05 g of the fine powder sediment (<63 μm) was weighed and put into a Teflon vessel. After that, 1.5 mL of mixed acid (2.5 HF : 3 HNO3 : 3 HCl) was added into the Teflon vessels using a single channel pipette, 100–1000 microlitre (μL) of the brand CappAero which was ISO 9001; 2000 certified. This digestion method is also known as the aqua digestion method, which was also applied by Chen and Ma [12] and Deely and Fergusson [13]. Finally, the Teflon Bomb jackets were screwed tightly to prevent the appearance of silicate gel on their bodies, before placing the Teflon bombs into the oven for 6 hours at 160°C. After 6 hours, they were cooled down under room temperature, where after that, 3.0 mL of acid solution composed of ethylenediaminetetraacetic acid (EDTA) and boric acid was added. The samples were then again put into the oven at 160°C for another 6 hours. The clear solution obtained was transferred into centrifuge tubes and meshed up to 10 mL with Mili-Q water. To verify the precision of the analytical procedures, the sediment samples were analysed in three replicates for each sampling point and a sample blank. To confirm analytical accuracy, portions of certified reference materials (SRM1646a-estuarine sediments) from the National Institute of Standards and Technology (NIST) were analysed with each batch of samples. The concentrations of metals (Cd, Co, Fe, Pb, and Zn) in the final digested solutions were then analysed using the Inductively Coupled Plasma Mass Spectrometer (Perkin Elmer. Elan 9000).

2.3. Ecological Risk Assessment

In this study, Fe is used as the normalized metal as it is an acceptable normalization element to be used in the calculation of enrichment factor since Fe distribution was not related to other heavy metals [13]. Fe usually has a relatively high natural concentration and is therefore not expected to be substantially enriched from anthropogenic sources in estuarine sediments [14]. These facts are in accordance with Daskalakis and O’Connor [15] who proposed that Fe is associated with fine solid surfaces, where its geochemistry is similar to that of many trace metals and its natural sediment concentration tends to be uniform. Moreover, a wide range of studies have used Fe and Al normalizations as an alternative to grain-size normalization [16, 17]. The enrichment factors (EFs) for each metal were calculated based on the formula following [1820]:

Meanwhile, the values for the earth’s crustal elements were taken from Carmichael  [21], Bodek et al. [22], and Ronov and Yaroshevsky [23], as published by Lide [24]. The resulting values were then referred to the contaminant categories proposed by Sutherland [25] to reveal and acknowledge the enrichment degree of the heavy metals as illustrated by Table 2.

tab2
Table 2: Five contaminant categories based on the EF value [25].

3. Result and Discussion

For method validation, certified reference material (SRM1646a) was determined as a precision check. The percentage of recoveries ( for each metal) for certified and measured concentration of those metals was satisfactory, with the recoveries being 81.67–102.24%. Table 3 shows the recovery test results for SRM (1646a) analysis.

tab3
Table 3: Recovery test results (concentration for Fe is in percentage (%), while other metals are in μg/g dry weight).

From this study, the heavy metal contents of the sediment were analysed, and the results were depicted in Table 4. The trend of mean concentration of heavy metals in Sungai Kilim was . According to Table 4, the concentration of Fe along the stations increased toward the upstream area, with the average value of %. The maximum and minimum concentration of Fe were obtained from St.5 and St.1 with the values 5.230% and 4.086% respectively. Furthermore, the highest concentration of Zn was found at St.6 with a value of 74.923 μg/g dry weight, whereas the lowest concentration was found at St.1 with a concentration of 59.81 μg/g dry weight. As for Zn, μg/g dry weight of Zn has been found distributed along Sungai Kilim. In the meantime, Co and Pb displayed the highest value at St.2 (8.872 μg/g dry weight) and St.4 (11.69 μg/g dry weight), correspondingly. Lastly, Cd was distributed unevenly along the river with the range value of 0.026–0.452 μg/g dry weight. According to the conducted statistical analysis of 2-way ANOVA, there was a significant difference between the sampling stations ().

tab4
Table 4: Heavy metal in each station (concentration for Fe is depicted in percentage (%), while other metals are in μg/g dry weight).

According to Rickard and Niagru [26] and Muller [27], the high concentration of heavy metals is usually not only influenced by the chemical processes but also by anthropogenic activities. Hence, these anthropogenic activities carried along the river plus the increase of development in that area are seen to have impacted the river as the domestic sewage, oil spill, and aquaculture, and many more are considered to be common sights. On average, Fe concentration in Sungai Kilim was lower compared to the earth’s crust value (5.635%). It was found that Fe was more concentrated at St.5 rather than the downstream, which could be possibly caused by the weathering process of sedimentary rocks that enter the river. Williamson [28] had stated that typical levels for Fe in sedimentary rocks are given as limestone 0.33%, sandstone 0.98%, shale 4.7%, and banded iron formation 28%. The distribution of Fe was rather uniform along the river, decreasing towards downstream. This trend suggests that only a small amount of Fe has been drifting downstream.

From the observation, the highest Zn concentration value was discovered at St.6 (upstream) and constantly decreasing towards the downstream area. This might be due to the bat waste from the bat cave at the upstream area. According to Miko et al. [29], bat droppings contain a relatively high content of Zn; thus, this could justify the source of Zn in this area. In addition, Davis et al. [30] showed that urban runoff might be slightly smaller. This is in agreement with Roney et al. [31] who found that Zn and its compounds are present in the Earth's crust and in most rocks, certain minerals, and some carbonate sediments, and, as a result of weathering effects towards these materials, soluble compounds of zinc are formed and may be released to water [32]. On the other hand, Monaci and Bargagli [33] have also found that the possible source of Zn may also be from motor oil, grease, phosphate fertilizers, sewage sludge, transmission fluid, undercoating, asphalt, and concrete.

Meanwhile, the average concentration of both Co and Pb in this study area was discovered to be slightly lower than the earth’s crust value. Faroon et al. [34] stated that the primary anthropogenic sources of Co lie in phosphate fertilizers, which, in this study, could be due to bat waste and aquaculture activities. Naylor et al. [35] have reported that fish pellets also contain some cobalt elements. A very high concentration of Pb at St.4 revealed that the surroundings were heavily contaminated with leaded petrol stemmed from outboard boat engines from boating activities and places of anchored yacht that were very concentrated in that particular area compared to the other stations. The contamination of Pb has been the major attention of many researchers such as Lee et al. [36], Alloway [37], and Monaci and Bargagli [33], where they have confirmed that leaded fuel, automobile or motor exhaust, lubricating oil, and grease are the possible major sources of Pb.

Lastly, the Cd concentration for all sampling stations was dramatically higher than that of the earth’s crust value, except for St.4. From the observation, the highest concentration indicated that the main source of heavy metal enrichment came from anthropogenic elements deposited directly or indirectly by human activities such as boat cruising along the study area. According to Miko et al. [29], oil combustion from the boating activities was the main factor that influenced the increase of Cd. On the other hand, Dong et al. [38] stated that human activities including discharges of municipal wastewater, agriculture, mining, fossil fuels, and industrial wastewater are a major source of Cd contamination in the marine environment especially in estuaries. Cd however has a very low solubility in aqueous solution. It is readily adsorbed to suspended solids, where, after a series of natural processes, Cd particles will finally sink and accumulate in sediments [39].

The comparison of heavy metal compositions made between the study area and other places in Malaysia is available in Table 5. The table shows the value of heavy metal concentrations reported by previous researchers, namely Kamaruzzaman and Ong [40], Jamil [10], Ahmad et al. [41], Kamaruzzaman et al. [42], Muhammad et al. [43] and Yap and Pang [44]. Based on Table 5, Zn concentration in the present study area was found to be relatively lower compared to the Juru area and earth bulk continental crust but higher compared to other places. Meanwhile, the other metals obtained seemed to have lower concentrations with respect to the other places in Malaysia.

tab5
Table 5: The comparison of heavy metal concentrations in the present study with other heavy metal studies throughout Malaysia.

For a better estimation of anthropogenic input, an enrichment factor (EF) was calculated for each metal by dividing its ration to the normalizing element by the same ration found in the chosen baseline. Generally, throughout this study, it was found that Fe distribution was rather uniform along the river, which indicates a good stability of the metal in the river as a whole. The good stability of Fe along the river signifies and ascertains its capability to be adopted as the most suitable normalizing element for this study compared to other metals, particularly in this study area. According to Figure 2, the EF value of Cd () for all stations was higher than 2.0 except for St.4 and St.5. This means that a significant portion of heavy metals was provided by anthropogenic sources mainly from the oil combustion from boating activities. However, Co (), Pb (), and Zn () obtained minimal enrichment (<2.0) for all stations. The data obtained will provide scientific evidence and may be referred to as the baseline data for a better understanding of our estuary ecosystem. In Malaysia, especially in Langkawi area, studies regarding heavy-metal pollution are currently quite limited. Therefore, continuous monitoring of heavy-metal pollution in this environment is definitely indispensable.

482451.fig.002
Figure 2: Enrichment factors for each heavy metal in the study area.

4. Conclusion

From this study, the overall trend of mean concentration of heavy metals in Sungai Kilim can be concluded by the following order: . However, the distribution of Cd in the sediment was found to be higher than that of the earth’s crust value, while other metals (Co, Fe, Pb, and Zn) were found to be of lower values. To clarify and validate how serious the contamination level of each metal to the river is, the enrichment factor (EF) was applied. According to this approach, the river was moderately enriched by Cd. The higher concentrations of Cd in the study area were estimated to have originated from the oil combustion of boating activities along the study area.

Acknowledgments

This research was conducted under the funding of University Teknologi MARA (UiTM), through the Research Excellence Fund. The authors wish to express their gratitude to the Oceanography Laboratory team members for their invaluable assistance and hospitality throughout the sampling period.

References

  1. A. M. Idris, “Combining multivariate analysis and geochemical approaches for assessing heavy metal level in sediments from Sudanese harbors along the Red Sea coast,” Microchemical Journal, vol. 90, no. 2, pp. 159–163, 2008. View at Publisher · View at Google Scholar · View at Scopus
  2. A. Demirak, F. Yilmaz, A. Levent Tuna, and N. Ozdemir, “Heavy metals in water, sediment and tissues of Leuciscus cephalus from a stream in southwestern Turkey,” Chemosphere, vol. 63, no. 9, pp. 1451–1458, 2006. View at Publisher · View at Google Scholar · View at Scopus
  3. J. M. Casas, H. Rosas, M. Solé, and C. Lao, “Heavy metals and metalloids in sediments from the Llobregat basin, Spain,” Environmental Geology, vol. 44, no. 3, pp. 325–332, 2003. View at Publisher · View at Google Scholar · View at Scopus
  4. K. P. Singh, D. Mohan, V. K. Singh, and A. Malik, “Studies on distribution and fractionation of heavy metals in Gomti river sediments—a tributary of the Ganges, India,” Journal of Hydrology, vol. 312, no. 1–4, pp. 14–27, 2005. View at Publisher · View at Google Scholar · View at Scopus
  5. J. Mwamburi, “Variations in trace elements in bottom sediments of major rivers in Lake Victoria's basin, Kenya,” Lakes and Reservoirs: Research and Management, vol. 8, no. 1, pp. 5–13, 2003. View at Publisher · View at Google Scholar · View at Scopus
  6. A. Tessier and P. G. C. Campbell, “Partitioning of trace metals in sediments: relationships with bioavailability,” Hydrobiologia, vol. 149, no. 1, pp. 43–52, 1987. View at Publisher · View at Google Scholar · View at Scopus
  7. N. W. Chan, “Protecting and conserving our natural heritage: potentials, threats and challenges of Langkawi Geopark,” in Proceedings of the International Conference World Civic Forum, Seoul, South Korea, May 2009.
  8. S. Tsugonai and M. Yamada, “226Ra in Bering sea sediment and its application as a geochronometer,” Geochemical Journal, vol. 13, pp. 231–238, 1980.
  9. B. Y. Kamaruzzaman, Geochemistry or the marine sediments. Its paleoceanographic significance [Ph.D. thesis], Hokkaido University, 1999.
  10. T. Jamil, Physicochemical and sediment characteristics of the bottom sediment of Terengganu River, Terengganu Malaysia [M.S. thesis], Kolej Universiti Sains dan Teknologi Malaysia, 2006.
  11. D. L. Trimm, H. H. Beiro, and S. J. Parker, “Comparison of digestion techniques in analyses for total metals in marine sediments,” Bulletin of Environmental Contamination and Toxicology, vol. 60, no. 3, pp. 425–432, 1998. View at Publisher · View at Google Scholar · View at Scopus
  12. M. Chen and L. Q. Ma, “Comparison of three aqua regia digestion methods for twenty Florida soils,” Soil Science Society of America Journal, vol. 65, no. 2, pp. 491–499, 2001. View at Scopus
  13. J. M. Deely and J. E. Fergusson, “Heavy metal and organic matter concentrations and distributions in dated sediments of a small estuary adjacent to a small urban area,” The Science of the Total Environment, vol. 153, no. 1-2, pp. 97–111, 1994. View at Publisher · View at Google Scholar · View at Scopus
  14. L. F. Niencheski, H. L. Windom, and R. Smith, “Distribution of particulate trace metal in Patos Lagoon estuary (Brazil),” Marine Pollution Bulletin, vol. 28, no. 2, pp. 96–102, 1994. View at Publisher · View at Google Scholar · View at Scopus
  15. K. D. Daskalakis and T. P. O'Connor, “Normalization and elemental sediment contamination in the coastal United States,” Environmental Science and Technology, vol. 29, no. 2, pp. 470–477, 1995. View at Scopus
  16. V. T. Breslin and S. A. Sañudo-Wilhelmy, “High spatial resolution sampling of metals in the sediment and water column in Port Jefferson Harbor, New York,” Estuaries, vol. 22, no. 3, pp. 669–680, 1999. View at Scopus
  17. H. L. Windom, S. J. Schropp, F. D. Calder et al., “Natural trace metal concentrations in estuarine and coastal marine sediments of the Southeastern United States,” Environmental Science and Technology, vol. 23, no. 3, pp. 314–320, 1989. View at Scopus
  18. S. Covelli and G. Fontolan, “Application of a normalization procedure in determining regional geochemical baselines,” Environmental Geology, vol. 30, no. 1-2, pp. 34–45, 1997. View at Publisher · View at Google Scholar · View at Scopus
  19. V. Simeonov, D. L. Massart, G. Andreev, and S. Tsakovski, “Assessment of metal pollution based on multivariate statistical modeling of “hot spot” sediments from the Black Sea,” Chemosphere, vol. 41, no. 9, pp. 1411–1417, 2000. View at Publisher · View at Google Scholar · View at Scopus
  20. L. Zhang, X. Ye, H. Feng et al., “Heavy metal contamination in western Xiamen Bay sediments and its vicinity, China,” Marine Pollution Bulletin, vol. 54, no. 7, pp. 974–982, 2007. View at Publisher · View at Google Scholar · View at Scopus
  21. R. S. Carmichael, CRC Practical Handbook of Physical Properties of Rocks and Minerals, CRC Press, Boca Raton, Fla, USA, 1989.
  22. I. Bodek, W. J. Lyman, W. F. Reehl, and D. H. Rosenblatt, Environmental Inorganic Chemistry, Pergamon Press, New York, NY, USA, 1988.
  23. A. B. Ronov and A. A. Yaroshevsky, “Earth's crust geochemistry,” in Encyclopedia of Geochemistry and Environmental Sciences, R. W. Fairbridge, Ed., Van Nostrand, New York, NY, USA, 1969.
  24. D. R. Lide, CRC Handbook of Chemistry and Physics, CRC Press, Boca Raton, Fla, USA, 85th edition, 2005.
  25. R. A. Sutherland, “Bed sediment-associated trace metals in an urban stream, Oahu, Hawaii,” Environmental Geology, vol. 39, no. 6, pp. 611–627, 2000. View at Scopus
  26. D. T. Rickard and J. O. Niagru, “Aqueous environmental chemistry of lead,” in The Biochemistry of Lead in the Environment: Part A. Ecological Cycles, pp. 219–284, Elsevier, Amsterdam, The Netherlands, 1978.
  27. F. L. L. Muller, “Colloid/solution partitioning of metal-selective organic ligands, and its relevance to Cu, Pb and Cd cycling in the firth of Clyde,” Estuarine, Coastal and Shelf Science, vol. 46, no. 3, pp. 419–437, 1998. View at Publisher · View at Google Scholar · View at Scopus
  28. M. A. Williamson, “Iron,” in Encyclopedia of Geochemistry, C. P. Marshall and R. W. Fairbridge, Eds., pp. 348–353, Kluwer Academic, Dordrecht, Germany, 1999.
  29. S. Miko, M. Kuhta, and S. Kapelj, “Environmental baseline geochemistry of sediments and percolating waters in the Modric Cave, Croatia,” Acta Carsologica, vol. 31, no. 1, pp. 135–149, 2002.
  30. A. P. Davis, M. Shokouhian, and S. Ni, “Loading estimates of lead, copper, cadmium, and zinc in urban runoff from specific sources,” Chemosphere, vol. 44, no. 5, pp. 997–1009, 2001. View at Publisher · View at Google Scholar · View at Scopus
  31. N. Roney, V. Cassandra, M. Williams, M. Osier, and S. J. Paikoff, Toxicological Profile for Zinc, U.S. Department Of Health And Human Services Public Health Service Agency for Toxic Substances and Disease Registry, 2005.
  32. NAS, “Inorganic solutes,” in Drinking Water and Health, vol. 1, pp. 205–229, National Academy of Sciences; National Academy Press, Washington, DC, USA, 1977.
  33. F. Monaci and R. Bargagli, “Barium and other trace metals as indicators of vehicle emissions,” Water, Air, and Soil Pollution, vol. 100, no. 1-2, pp. 89–98, 1997. View at Scopus
  34. O. M. Faroon, H. Abadin, S. Keith et al., Toxicological Profile for Cobalt, U.S. Department of Health and Human Services Public Health Service Agency for Toxic Substances and Disease Registry, 2004.
  35. S. J. Naylor, R. D. Moccia, and G. M. Durant, “The chemical composition of settleable solid fish waste (Manure) from commercial rainbow trout farms in Ontario, Canada,” North American Journal of Aquaculture, vol. 61, no. 1, pp. 21–26, 1999. View at Scopus
  36. D. S. Lee, J. A. Garland, and A. A. Fox, “Atmospheric concentrations of trace elements in urban areas of the United Kingdom,” Atmospheric Environment, vol. 28, no. 16, pp. 2691–2713, 1994. View at Publisher · View at Google Scholar · View at Scopus
  37. B. J. Alloway, Heavy Metals in Soils, Blackie Academic and Professional, Glasgow, UK, 2nd edition, 1995.
  38. C. D. Dong, C. F. Chen, M. S. Ko, and C. W. Chen, “Enrichment, accumulation and ecological risk evaluation of cadmium in the surface sediments of Jen-Gen River Estuary, Taiwan,” International Journal of Chemical Engineering and Applications, vol. 3, no. 6, pp. 370–373, 2012.
  39. C. W. Chen, C. F. Chen, and C. D. Dong, “Contamination and potential ecological risk of mercury in sediments of Kaohsiung River mouth, Taiwan,” International Journal of Environmental Science and Development, vol. 3, pp. 66–71, 2012.
  40. Y. Kamaruzzaman and M. C. Ong, “Geochemical proxy of some chemical elements in sediments of kemaman river Estuary, Terengganu, Malaysia,” Sains Malaysiana, vol. 38, no. 5, pp. 631–636, 2009. View at Scopus
  41. A. K. Ahmad, I. Mushrifah, and M. Shuhaimi-Othman, “Water quality and heavy metal concentrations in sediment of Sungai Kelantan, Kelantan, Malaysia: a baseline study,” Sains Malaysiana, vol. 38, no. 4, pp. 435–442, 2009. View at Scopus
  42. B. Y. Kamaruzzaman, N. T. Shuhada, B. Akbar et al., “Spatial concentrations of lead and copper in bottom sediments of Langkawi Coastal Area, Malaysia,” Research Journal of Environmental Sciences, vol. 5, pp. 179–186, 2011. View at Publisher · View at Google Scholar
  43. B. G. Muhammad, N. A. S. Wan, and I. Mohd, “Sebaran logam berat dalam lembangan sungai semenyih,” in Proceedings of the Regional Symposium on Environment and Natural Resources, vol. 1, pp. 595–602, Kuala Lumpur, Malaysia, April 2002.
  44. C. K. Yap and B. H. Pang, “Assessment of Cu, Pb, and Zn contamination in sediment of north western Peninsular Malaysia by using sediment quality values and different geochemical indices,” Environmental Monitoring and Assessment, vol. 183, no. 1–4, pp. 23–39, 2011. View at Publisher · View at Google Scholar · View at Scopus