Reduced Formation of Oxidative Stress Biomarkers and Migration of Mononuclear Phagocytes in the Cochleae of Chinchilla after Antioxidant Treatment in Acute Acoustic Trauma
Figure 3
CD45+ immunostaining and immunostaining density in the stria vascularis. Examples of CD45+ immunostaining images obtained from the stria vascularis in the middle turn of cochleae from the normal control (a), noise exposure (b), and noise/treatment (c) groups by light microscopy. Few CD45+ cells were found in the stria vascularis of the normal group (arrows in (a)). There was a large increase in the number of CD45+ cells in the stria vascularis of the noise exposure group (arrows in (b)). The antioxidant treatment significantly decreased the number of CD45+ cells in the stria vascularis (arrows in (c)). CD45+ cells were also found in the spiral ligament of the noise and the noise/treatment groups (arrowheads in b and c), but not in the normal control group. The results of the immunostaining density measurement are shown in (d) (number of cochleae = 6 in each group). There were significant differences among the three groups and in pairwise comparisons in the three groups in each turn. The numbers (1, 2, 3) in (d) indicate the normal control, noise exposure, and noise/treatment groups, respectively. Scale bar = 20 μm in (c) for (a)–(c) (error bars represent standard error of the means. *, **, *** indicates , 0.001, resp. Number of cochleae = 6 in each group).
