Reduced Formation of Oxidative Stress Biomarkers and Migration of Mononuclear Phagocytes in the Cochleae of Chinchilla after Antioxidant Treatment in Acute Acoustic Trauma
Figure 4
4-HNE immunostaining and relative fluorescence intensity in the organ of Corti. Examples of 4-HNE immunostaining images obtained from the organ of Corti in the middle turn of cochleae from the normal control (a), noise exposure (b) and noise/treatment (c) groups by confocal microscopy using the same microscopic and image collecting settings. No significant 4-HNE staining was found in the organ of Corti of the normal control group (a). Strong positive staining was seen in IHC (arrow in (b)), inner and outer pillar cells (open arrowheads in (b)), Deiters cells (arrowheads), and cells of Hensen (starburst) and Boettcher of the noise exposure group. The OHC region also had positive, but relatively weak 4-HNE immunostaining (bracket in B). Significantly lower HNE staining was found in the organ of Corti in the noise/treatment group (c). The results of the relative fluorescence intensity in the organ of Corti measured by LAS AF Lite software are shown in (d). High fluorescence intensity was found only in the organ of Corti of the noise exposure group. There were significant differences among three groups and between the normal control and the noise exposure groups, as well as between the noise exposure and the noise/treatment groups (***indicates ). However, there was no significant difference between the normal control and the noise/treatment groups (). There was no significant difference among three turns within each group (). The numbers (1, 2, 3) in (d) indicate the normal control, noise exposure and noise/treatment groups, respectively. Brackets indicate the OHC region in (a)–(c). Scale bar = 20 μm in (c) for (a)–(c) (Error bars represent standard error of the means. Number of cochleae = 6 in each group).
