Schwann Cell Metabolic Activity in Various Short-Term Holding Conditions: Implications for Improved Nerve Graft Viability
Figure 2
This Figure and respectively, Figure 3: Schwann cell metabolism indicated as fluorescence activity at 4°C (a), room temperature (b), and at 37°C (in (c)) incubated in the described media. Cell viability was measured by reduction of resazurin to resofurin by CellTiter-Blue (CTB) Cell Viability Assay. Most reduced cell viability was observed after 3-hour incubation of Schwann cells in DMEM at 4°C. Optimal cell viability with increase of Schwann cell metabolism can be seen after incubation in HBRM at 37°C. .
